MixedLow evidencePaper 54

Blueberry anthocyanins regulate SIRT1/FoxO1 pathway to inhibit oxidative stress and reduce

Keywords

#microwave radiation #2.856 GHz #testis #male reproductive system #oxidative stress #blueberry anthocyanins #SIRT1 #FoxO1 #sperm motility #sex hormones #GSH/GSSG #SOD #MDA #LDH #SDH #ATP synthase #Caspase-3 #apoptosis

Study snapshot

PublicationJournal of Functional Foods · 2024

Study typeanimal

Reported directionMixed

Evidence ratingLow

Abstract

Blueberry anthocyanins regulate SIRT1/FoxO1 pathway to inhibit oxidative stress and reduce testicular tissue damage induced by microwave radiation in rats Pang Y, Men J, Li Y, Zhang J, Zhao L, et al. Blueberry anthocyanins regulate SIRT1/FoxO1 pathway to inhibit oxidative stress and reduce testicular tissue damage induced by microwave radiation in rats. Journal of Functional Foods. Vol. 122, 2024. doi: 10.1016/j.jff.2024.106523. Abstract Researches have shown that microwave radiation could cause oxidative stress injury in male reproductive system, and blueberry anthocyanins had excellent oxidation resistance. Our study aimed to investigate the protective effect of blueberry anthocyanins (100, 200 and 400 mg/kg/d) on testicular tissue damage in Wistar rats exposed to 2.856 GHz microwave and the optimal dose. We found that blueberry anthocyanins could ameliorate the decrease of sperm motility and sex hormone levels and testicular tissue structure damage caused by microwave radiation, increase SIRT1 expression and decrease FoxO1 expression, increase GSH/GSSG, SOD and inhibit MDA. The LDH, SDH and ATP synthase were increased, and Caspase-3 expression was decreased, and the high-dose of blueberry anthocyanins (400 mg/kg/d) had the best protective effect. These results suggested that blueberry anthocyanins could inhibit oxidative stress injury induced by 2.856 GHz microwave radiation in rat testicular tissue by regulating SIRT1/FoxO1 pathway, enhance energy metabolism and reduce cell apoptosis. Open access paper: sciencedirect.com

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal

Effect directionmixed

PopulationWistar rats

Sample sizeNot supplied

Exposuremicrowave · 2856 MHz

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

In Wistar rats exposed to 2.856 GHz microwave radiation, blueberry anthocyanins (100, 200, 400 mg/kg/d) were reported to ameliorate decreases in sperm motility and sex hormone levels and reduce testicular tissue structural damage. Anthocyanins were associated with increased SIRT1 expression, decreased FoxO1 expression, improved oxidative stress markers (increased GSH/GSSG and SOD; inhibited MDA), increased LDH/SDH/ATP synthase, and decreased Caspase-3 expression; 400 mg/kg/d showed the best protective effect.

Outcomes measured

Testicular tissue damage/structure
Sperm motility
Sex hormone levels
Oxidative stress markers (GSH/GSSG, SOD, MDA)
SIRT1 expression
FoxO1 expression
Energy metabolism markers (LDH, SDH, ATP synthase)
Apoptosis marker (Caspase-3 expression)

Limitations

Sample size not reported in the provided abstract/metadata
Exposure duration and exposure metrics beyond frequency (e.g., SAR/power density) not reported
Details of randomization/blinding and control conditions not reported
Findings are from an animal model; human relevance not established in the provided text

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": "microwave",
    "source": null,
    "frequency_mhz": 2856,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Wistar rats",
  "sample_size": null,
  "outcomes": [
    "Testicular tissue damage/structure",
    "Sperm motility",
    "Sex hormone levels",
    "Oxidative stress markers (GSH/GSSG, SOD, MDA)",
    "SIRT1 expression",
    "FoxO1 expression",
    "Energy metabolism markers (LDH, SDH, ATP synthase)",
    "Apoptosis marker (Caspase-3 expression)"
  ],
  "main_findings": "In Wistar rats exposed to 2.856 GHz microwave radiation, blueberry anthocyanins (100, 200, 400 mg/kg/d) were reported to ameliorate decreases in sperm motility and sex hormone levels and reduce testicular tissue structural damage. Anthocyanins were associated with increased SIRT1 expression, decreased FoxO1 expression, improved oxidative stress markers (increased GSH/GSSG and SOD; inhibited MDA), increased LDH/SDH/ATP synthase, and decreased Caspase-3 expression; 400 mg/kg/d showed the best protective effect.",
  "effect_direction": "mixed",
  "limitations": [
    "Sample size not reported in the provided abstract/metadata",
    "Exposure duration and exposure metrics beyond frequency (e.g., SAR/power density) not reported",
    "Details of randomization/blinding and control conditions not reported",
    "Findings are from an animal model; human relevance not established in the provided text"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "microwave radiation",
    "2.856 GHz",
    "testis",
    "male reproductive system",
    "oxidative stress",
    "blueberry anthocyanins",
    "SIRT1",
    "FoxO1",
    "sperm motility",
    "sex hormones",
    "GSH/GSSG",
    "SOD",
    "MDA",
    "LDH",
    "SDH",
    "ATP synthase",
    "Caspase-3",
    "apoptosis"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/j.jff.2024.106523
PubMed: Not supplied

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